Ligation of PCR Products
This video is a description of a process called ligation. It's intended to be a pre-lab background before actually performing a ligation. There's also a discussion of the necessity of blunting the PCR product prior to...
This video is a description of a process called ligation. It's intended to be a pre-lab background before actually performing a ligation. There's also a discussion of the necessity of blunting the PCR product prior to ligation. Ligation of PCR products into plasmid vectors is a critical step prior to transformation. Plasmids that contain inserts will then be used to transform bacteria for cloning.
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